c-Cbl Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

c-Cbl Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 25818-1-AP

Synonyme

CBL, C CBL, Casitas B-lineage lymphoma proto-oncogene, CBL2, E3 ubiquitin-protein ligase CBL

---

• 

  WB analysis using 25818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25818-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 25818-1-AP

  IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.

• 

  IP experiment of K-562 using 25818-1-AP

  IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.

• 

  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below

Produktinformation

25818-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA c-Cbl und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: c-Cbl fusion protein Ag23033
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC132733
• Gene symbol: CBL
• Gene ID (NCBI): 867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Publikationen

Species	Application	Title
human	WB,IF	Hepatology; PMID: 29729186; Authors - Huixia Zhang ; KD Validated
human	WB,IF,CoIP	Cell Rep; Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis; Authors - Huan Li ; KD Validated
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	WB	Cell Commun Signal; Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells; Authors - Limei Xu
mouse	WB	Front Med; Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ; Authors - Fei Xu
human	WB	Oncogene; AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression; Authors - Sicheng Wan