c-Cbl Monoklonaler Antikörper

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• KD/KO Validated

c-Cbl Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2F6D1

Rezensionen (1)

Kat-Nr. : 66576-1-Ig

Synonyme

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

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Galerie der Validierungsdaten

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  WB analysis using 66576-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66576-1-Ig (c-Cbl antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66576-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66576-1-Ig (CBL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66576-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66576-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Daudi-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

66576-1-Ig bindet in WB, IHC, ELISA c-Cbl und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: c-Cbl fusion protein Ag23033
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC132733
• Gene symbol: CBL
• Gene ID (NCBI): 867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Publikationen

Species	Application	Title
human	WB	Autophagy; RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.; Authors - Weijie Dong ; KD Validated
human	WB	Cytokine; The CBL-LSD1-CXCL8 axis regulates methionine metabolism in glioma.; Authors - Jie Chang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kus (Verified Customer) (12-08-2022)

This Ab always worked perfect for our experiments in western blotting at 1:1000 or 2000 dilution in 5% BSA+TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

c-Cbl Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:66576-1-Ig)