CBLB Monoklonaler Antikörper

CBLB Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D3A2

Kat-Nr. : 66353-1-PBS

Synonyme

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

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  WB analysis of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 66353-1-Ig (same clone as 66353-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66353-1-PBS bindet in WB, IHC, IF/ICC, ELISA CBLB und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CBLB fusion protein Ag18757
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Berechnetes Molekulargewicht: 982 aa, 109 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC032851
• Gene symbol: CBLB
• Gene ID (NCBI): 868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.