CBLC Polyklonaler Antikörper

CBLC Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13104-1-AP

Synonyme

CBL 3, CBL SL, CBL3, CBLC, RING finger protein 57, RNF57, SH3 binding protein CBL 3, SH3 binding protein CBL C

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 13104-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13104-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13104-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13104-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13104-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13104-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13104-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13104-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13104-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13104-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 13104-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 13104-1-AP (CBLC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HaCaT-Zellen, HepG2-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13104-1-AP bindet in WB, ELISA CBLC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBLC fusion protein Ag3747
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence c
• Berechnetes Molekulargewicht: 474 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC028915
• Gene symbol: CBLC
• Gene ID (NCBI): 23624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	PLoS One; Loss of function Cbl-c mutations in solid tumors.; Authors - Silvano Rakeem Daniels