CBLL1 Polyklonaler Antikörper

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• KD/KO Validated

CBLL1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21179-1-PBS

Synonyme

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  WB analysis using 21179-1-AP (same clone as 21179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21179-1-AP (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 21179-1-AP (same clone as 21179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21179-1-AP (same clone as 21179-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody (21179-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21179-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CBLL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBLL1 fusion protein Ag15463
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
• Berechnetes Molekulargewicht: 491 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC027460
• Gene symbol: CBLL1
• Gene ID (NCBI): 79872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)