CBR3 Polyklonaler Antikörper

CBR3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15619-1-AP

Synonyme

NADPH-dependent carbonyl reductase 3, hCBR3, EC:1.6.5.10, EC:1.1.1.184, carbonyl reductase 3

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  WB analysis using 15619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15619-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15619-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15619-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15619-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15619-1-AP (CBR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

15619-1-AP bindet in WB, IHC, IF/ICC, ELISA CBR3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBR3 fusion protein Ag8039
• Vollständiger Name: carbonyl reductase 3
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC002812
• Gene symbol: CBR3
• Gene ID (NCBI): 874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CBR3(Carbonyl reductase [NADPH] 3), which Belongs to the short-chain dehydrogenases/reductases (SDR) family, has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). The gene encodes a 31 kDa protein that can catalyze the reduction of many endogenous and xenobiotic carbonyl compounds, including steroids and prostaglandins. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein(PMID:9740676).

Publikationen

Species	Application	Title
human,mouse	WB	Antioxid Redox Signal; Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.; Authors - Maya A Olshina
mouse	WB	Carcinogenesis; Thioredoxin reductase 1 protects against chemically induced hepatocarcinogenesis via control of cellular redox homeostasis.; Authors - Carlson Bradley A BA
rat	WB	Nat Commun; Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family; Authors - Fanindra Kumar Deshmukh
human	WB,IHC	World Allergy Organ J; Integrated machine learning and bioinformatic analysis of mitochondrial-related signature in chronic rhinosinusitis with nasal polyps; Authors - Bo Yang