CBR3 Polyklonaler Antikörper

CBR3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15619-1-PBS

Synonyme

---

• 

  WB analysis using 15619-1-AP (same clone as 15619-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15619-1-AP (same clone as 15619-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15619-1-AP (same clone as 15619-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15619-1-AP (CBR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

15619-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CBR3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBR3 fusion protein Ag8039
• Vollständiger Name: carbonyl reductase 3
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC002812
• Gene symbol: CBR3
• Gene ID (NCBI): 874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CBR3(Carbonyl reductase [NADPH] 3), which Belongs to the short-chain dehydrogenases/reductases (SDR) family, has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). The gene encodes a 31 kDa protein that can catalyze the reduction of many endogenous and xenobiotic carbonyl compounds, including steroids and prostaglandins. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein(PMID:9740676).