CBX3 Monoklonaler Antikörper

CBX3 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1A5A3

Rezensionen (1)

Kat-Nr. : 66446-1-Ig

Synonyme

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

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  WB analysis of NIH/3T3 using 66446-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66446-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66446-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66446-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66446-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66446-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66446-1-Ig bindet in WB, IF/ICC, ELISA CBX3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CBX3 fusion protein Ag19112
• Vollständiger Name: chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000954
• Gene symbol: CBX3
• Gene ID (NCBI): 11335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Publikationen

Species	Application	Title
human	WB	Int J Biochem Cell Biol; NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.; Authors - Dan Wang
human	WB	Adv Biol (Weinh); CBX3 Downregulates HLTF to Activate PI3K/AKT Signaling Promoting Cholangiocarcinoma; Authors - Min Xie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

ILIAS (Verified Customer) (05-26-2023)

The 66446-1-Ig worked very well in both Western Blot and Immunofluorescence microscopy experiments

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB), 1:500(IF)
• Cell Tissue Type: HeLa and MCF7 cells

CBX3 Antibody Western Blot,Immunofluorescence validation (1:2000 (WB), 1:500(IF) dilution) in HeLa and MCF7 cells (Cat no:66446-1-Ig)