CBX3 Monoklonaler Antikörper

CBX3 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5A3

Kat-Nr. : 66446-1-PBS

Synonyme

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

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  WB analysis of NIH/3T3 using 66446-1-Ig (same clone as 66446-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66446-1-Ig (same clone as 66446-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66446-1-Ig (same clone as 66446-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66446-1-Ig (same clone as 66446-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66446-1-Ig (same clone as 66446-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66446-1-Ig (same clone as 66446-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66446-1-PBS bindet in WB, IF/ICC, Indirect ELISA CBX3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CBX3 fusion protein Ag19112
• Vollständiger Name: chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000954
• Gene symbol: CBX3
• Gene ID (NCBI): 11335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins