CBX4 Polyklonaler Antikörper

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• KD/KO Validated

CBX4 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, PLA, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18544-1-AP

Synonyme

CBX4, Chromobox protein homolog 4, E3 SUMO protein ligase CBX4, hPC2, NBP16, PC2, Polycomb 2 homolog

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 cell using 18544-1-AP

  WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).

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  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS cell using 18544-1-AP

  IF result of anti-CBX4 (18544-1-AP, 1:200) with U2OS cell, MeOH fixation. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).

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  IF Staining of HeLa using 18544-1-AP

  Immunofluorescent analysis of HeLa cells using 18544-1-AP (CBX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Various cell, PC-3-Zellen
• Erfolgreiche Detektion in IF: U2OS cell, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

18544-1-AP bindet in WB, PLA, IF, ELISA CBX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBX4 fusion protein Ag13425
• Vollständiger Name: chromobox homolog 4 (Pc class homolog, Drosophila)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: ~75 kDa
• GenBank-Zugangsnummer: BC014967
• Gene symbol: CBX4
• Gene ID (NCBI): 8535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.

Publikationen

Species	Application	Title
human	WB,IF,PLA	Front Cell Dev Biol; SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.; Authors - Immacolata Giordano
human	WB	Bioengineered; Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.; Authors - Zengxi Yang ; KD Validated
human	WB	Mol Oncol; CBX4 promotes anti-tumor immunity by suppressing Pdcd1 expression in T cells; Authors - Liwei Ren