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CBX5 Polyklonaler Antikörper

CBX5 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31109-1-PBS

Synonyme

Antigen p25, HP1, HP1 alpha, HP1A

Filter:
  • Western Blot (WB) analysis of various lysates using CBX5 Polyclonal antibody (31109-1-AP)

    WB analysis using 31109-1-AP (same clone as 31109-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31109-1-AP (CBX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31109-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CBX5 Polyclonal antibody (31109-1-AP)

    WB analysis of K-562 using 31109-1-AP (same clone as 31109-1-PBS)

    WB result of CBX5 antibody (31109-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX5 transfected K-562 cells. This data was developed using the same antibody clone with 31109-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31109-1-PBS bindet in WB, Indirect ELISA CBX5 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CBX5 fusion protein Ag34810
Vollständiger Name chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Berechnetes Molekulargewicht 191 aa, 22 kDa
Beobachtetes Molekulargewicht25 kDa
GenBank-ZugangsnummerBC006821
Gene symbol CBX5
Gene ID (NCBI) 23468
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443). This antibody is a rabbit polyclonal antibody raised against a full-length human HP1A protein ,and can reacts with the 28kd HP1A protein.