CBX5 Rekombinanter Antikörper

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• KD/KO Validated

CBX5 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240145F11

Kat-Nr. : 83258-5-RR

Synonyme

240145F11, Antigen p25, HP1, HP1 alpha, HP1A

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  WB analysis of K-562 using 83258-5-RR

  WB result of CBX5 antibody (83258-5-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX5 transfected K-562 cells.

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  WB analysis using 83258-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83258-5-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 83258-5-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 83258-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-5-RR, Clone: 240145F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 83258-5-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug CBX5 Recombinant antibody (83258-5-RR, Clone:240145F11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83258-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83258-5-RR against Human CBX5 were performed. The affinity constant is 0.339 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maushirngewebe, Maus-Magengewebe, Rattennierengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83258-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA CBX5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CBX5 fusion protein Ag34810
• Vollständiger Name: chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• Berechnetes Molekulargewicht: 191 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC006821
• Gene symbol: CBX5
• Gene ID (NCBI): 23468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443).