CBX6 Polyklonaler Antikörper

CBX6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23426-1-AP

Synonyme

CBX6, chromobox homolog 6, Chromobox protein homolog 6

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 23426-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 23426-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23426-1-AP bindet in WB, IHC, ELISA CBX6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBX6 fusion protein Ag20014
• Vollständiger Name: chromobox homolog 6
• Berechnetes Molekulargewicht: 412 aa, 44 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC064900
• Gene symbol: CBX6
• Gene ID (NCBI): 23466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Chromobox domain (Cbx) gene family, consisting of Polycomb and Heterochromatin Protein 1 genes, involves in transcriptional repression, cell cycle regulation and chromatin remodeling [PMID: 17486619]. Cbx6, other named neuronal pentraxin receptor (NPR), first described as a putative integral membrane pentraxin that interacts with neuronal pentraxin 1 and 2, has also been classified as a Pc protein [PMID: 14647293]. NPR was determined to be a member of a distinct pentraxin protein subfamily, and found to form heteropentamer swithNP1 andNP2, followed by release from cell membranes [PMID: 10748068].

Publikationen

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang