CC2D1A Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CC2D1A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C11G2

Kat-Nr. : 68334-1-PBS

Synonyme

FREUD 1, AKI1, Akt kinase-interacting protein 1, Coiled-coil and C2 domain-containing protein 1A, Five prime repressor element under dual repression-binding protein 1

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  WB analysis using 68334-1-Ig (same clone as 68334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68334-1-Ig (CC2D1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68334-1-Ig (same clone as 68334-1-PBS)

  WB result of CC2D1A antibody (68334-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CC2D1A transfected HEK-293 cells. This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68334-1-Ig (same clone as 68334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68334-1-Ig (CC2D1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68334-1-Ig (same clone as 68334-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CC2D1A antibody (68334-1-Ig, Clone: 1C11G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68334-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CC2D1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CC2D1A fusion protein Ag10363
• Vollständiger Name: coiled-coil and C2 domain containing 1A
• Berechnetes Molekulargewicht: 951 aa, 104 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC064981
• Gene symbol: CC2D1A
• Gene ID (NCBI): 54862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.