CCAR1 Polyklonaler Antikörper

CCAR1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25232-1-PBS

Synonyme

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  WB analysis using 25232-1-AP (same clone as 25232-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25232-1-AP (CCAR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 25232-1-AP (same clone as 25232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 25232-1-AP (same clone as 25232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 25232-1-AP (same clone as 25232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25232-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CCAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCAR1 fusion protein Ag18970
• Vollständiger Name: cell division cycle and apoptosis regulator 1
• Berechnetes Molekulargewicht: 1150 aa, 133 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC132725
• Gene symbol: CCAR1
• Gene ID (NCBI): 55749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.