CCAR1 Rekombinanter Antikörper

CCAR1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240980C12

Kat-Nr. : 83936-4-PBS

Synonyme

240980C12, CARP 1, CARP1, CARP-1, Death inducer with SAP domain

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  WB analysis using 83936-4-RR (same clone as 83936-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83936-4-RR (CCAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83936-4-RR (CCAR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83936-4-RR (CCAR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83936-4-RR (same clone as 83936-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (83936-4-RR, Clone: 240980C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83936-4-PBS bindet in WB, IHC, IF/ICC, ELISA CCAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCAR1 fusion protein Ag18786
• Vollständiger Name: cell division cycle and apoptosis regulator 1
• Berechnetes Molekulargewicht: 1150 aa, 133 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC132725
• Gene symbol: CCAR1
• Gene ID (NCBI): 55749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.