CCAR2 Monoklonaler Antikörper

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• KD/KO Validated

CCAR2 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2D10F8

Kat-Nr. : CL594-66497

Synonyme

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967

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  IF Staining of HeLa using CL594-66497

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated CCAR2 antibody (CL594-66497, Clone: 2D10F8 ) at dilution of 1:100.

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  FC experiment of HeLa using CL594-66497

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of U2OS using CL594-66497

  1X10^6 U2OS cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: U2OS-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66497 bindet in IF/ICC, FC (Intra) CCAR2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CCAR2 fusion protein Ag18374
• Vollständiger Name: KIAA1967
• Berechnetes Molekulargewicht: 923 aa, 103 kDa
• GenBank-Zugangsnummer: BC018269
• Gene symbol: CCAR2
• Gene ID (NCBI): 57805
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).