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  • KD/KO Validated

CCDC111/PRIMPOL Polyklonaler Antikörper

CCDC111/PRIMPOL Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 29824-1-AP

Synonyme

CCDC111, FLJ33167, hPrimpol1, PRIMPOL



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCU2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

29824-1-AP bindet in WB, IHC, IF/ICC, ELISA CCDC111/PRIMPOL und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CCDC111/PRIMPOL fusion protein Ag31478
Vollständiger Name coiled-coil domain containing 111
Beobachtetes Molekulargewicht64 kDa
GenBank-ZugangsnummerBC064600
Gene symbol CCDC111/PRIMPOL
Gene ID (NCBI) 201973
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CCDC111/PRIMPOL antibody 29824-1-APProtokoll herunterladen
IHC protocol for CCDC111/PRIMPOL antibody 29824-1-APProtokoll herunterladenl
IF protocol for CCDC111/PRIMPOL antibody 29824-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Commun

An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress

Authors - Taichi Igarashi

Nat Commun

Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases

Authors - Anastasia Hale
humanWB

Int J Mol Sci

CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin

Authors - Nicole L Batenburg
humanWB

bioRxiv

CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps

Authors - Lindsey M Pale
humanWB

Nucleic Acids Res

USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress

Authors - Alexandra Nusawardhana
humanWB

Cell Rep

FANCM promotes PARP inhibitor resistance by minimizing ssDNA gap formation and counteracting resection inhibition

Authors - Zeyuan Liu
  • KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hasan (Verified Customer) (01-31-2023)

WB analysis of PrimPol in whole lysate of different clones of HeLa cells. Blocking was done for 1h at room temperature with 5% skimmed milk. The primary Ab was used with 1:2000 dilution overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa cells/ different clones
CCDC111/PRIMPOL Antibody Western Blot validation (1:2000 dilution) in HeLa cells/ different clones (Cat no:29824-1-AP)