CCDC111/PRIMPOL Polyklonaler Antikörper

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CCDC111/PRIMPOL Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29824-1-PBS

Synonyme

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  WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 29824-1-AP (same clone as 29824-1-PBS)

  WB result of CCDC111/PRIMPOL antibody (29824-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC111/PRIMPOL transfected HepG2 cells. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 29824-1-AP (same clone as 29824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29824-1-AP (same clone as 29824-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC111/PRIMPOL antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29824-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CCDC111/PRIMPOL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC111/PRIMPOL fusion protein Ag31478
• Vollständiger Name: coiled-coil domain containing 111
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC064600
• Gene symbol: CCDC111/PRIMPOL
• Gene ID (NCBI): 201973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).