CCDC12 Polyklonaler Antikörper

CCDC12 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25138-1-PBS

Synonyme

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  WB analysis of HeLa using 25138-1-AP (same clone as 25138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 25138-1-AP (same clone as 25138-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 25138-1-AP (same clone as 25138-1-PBS)

  IP result of anti-CCDC12 (IP:25138-1-AP, 4ug; Detection:25138-1-AP 1:600) with HeLa cells lysate 2560ug. This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 25138-1-AP (same clone as 25138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 25138-1-AP (same clone as 25138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25138-1-AP (same clone as 25138-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC12 antibody (25138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25138-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CCDC12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC12 fusion protein Ag18223
• Vollständiger Name: coiled-coil domain containing 12
• Berechnetes Molekulargewicht: 166 aa, 19 kDa
• Beobachtetes Molekulargewicht: 18-25 kDa
• GenBank-Zugangsnummer: BC020830
• Gene symbol: CCDC12
• Gene ID (NCBI): 151903
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC12 is a 166aa protein with MW 18-25 kDa (with some modification).