CCDC134 Rekombinanter Antikörper

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• KD/KO Validated

CCDC134 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241212F11

Kat-Nr. : 84106-3-PBS

Synonyme

241212F11

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• 

  WB analysis using 84106-3-RR (same clone as 84106-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84106-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 84106-3-RR (same clone as 84106-3-PBS)

  WB result of CCDC134 antibody (84106-3-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC134 transfected HEK-293 cells. This data was developed using the same antibody clone with 84106-3-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 84106-3-RR (same clone as 84106-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84106-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01057-1

  Cytometric bead array standard curve of MP01057-1, CCDC134 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84106-1-PBS. Detection antibody: 84106-3-PBS. Standard: Ag21990. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84106-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84106-3-RR against Human CCDC134 were performed. The affinity constant is 1.69 nM.

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Geprüfte Anwendungen

Produktinformation

84106-3-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA CCDC134 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCDC134 fusion protein Ag21990
• Vollständiger Name: coiled-coil domain containing 134
• Berechnetes Molekulargewicht: 229 aa, 27 kDa
• Beobachtetes Molekulargewicht: 25-27 kDa
• GenBank-Zugangsnummer: BC017693
• Gene symbol: CCDC134
• Gene ID (NCBI): 79879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.