CCDC22 Polyklonaler Antikörper

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• KD/KO Validated

CCDC22 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 16636-1-AP

Synonyme

JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22

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  WB analysis of human brain using 16636-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16636-1-AP

  WB result of CCDC22 antibody (16636-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.

• 

  WB analysis of rat colon using 16636-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16636-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16636-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16636-1-AP

  IP result of anti-CCDC22 (IP:16636-1-AP, 4ug; Detection:16636-1-AP 1:600) with mouse lung tissue lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Kolongewebe, Mauslungengewebe, Ratten-Kolongewebe
• Erfolgreiche IP: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

16636-1-AP bindet in WB, IP, CoIP, ELISA CCDC22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC22 fusion protein Ag9980
• Vollständiger Name: coiled-coil domain containing 22
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC000972
• Gene symbol: CCDC22
• Gene ID (NCBI): 28952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi
human	WB, IP	J Cell Biol; Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.; Authors - Haiying Li
human	WB	Mol Metab; Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.; Authors - Liu Yang