CCDC28A Polyklonaler Antikörper

CCDC28A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14807-1-AP

Synonyme

C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A

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  WB analysis using 14807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 14807-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

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  IF Staining of MCF-7 using 14807-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCDC28A antibody 14807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14807-1-AP bindet in WB, IF/ICC, ELISA CCDC28A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC28A fusion protein Ag6529
• Vollständiger Name: coiled-coil domain containing 28A
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC000758
• Gene symbol: CCDC28A
• Gene ID (NCBI): 25901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).