TACO1/CCDC44 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TACO1/CCDC44 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 21147-1-AP

Synonyme

CCDC44, TACO1, TACO1/CCDC44

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  WB analysis using 21147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21147-1-AP

  WB result of TACO1/CCDC44 antibody (21147-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TACO1/CCDC44 transfected HeLa cells.

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  IP experiment of mouse liver using 21147-1-AP

  IP result of anti-TACO1/CCDC44 (IP:21147-1-AP, 4ug; Detection:21147-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 21147-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21147-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 21147-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21147-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

21147-1-AP bindet in WB, IHC, IP, RIP, ELISA TACO1/CCDC44 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TACO1/CCDC44 fusion protein Ag15688
• Vollständiger Name: coiled-coil domain containing 44
• Berechnetes Molekulargewicht: 297 aa, 32 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC007744
• Gene symbol: TACO1
• Gene ID (NCBI): 51204
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TACO1 (translational activator of cytochrome oxidase subunit I) is required for COXI translation. Mutations in TACO1 cause Leigh syndrome (LS) and cytochrome c oxidase deficiency. (27319982)

Publikationen

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB	Nat Commun; Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.; Authors - Tara R Richman ; KO Validated
mouse	WB	EMBO J; Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.; Authors - Nicola Ferreira
human	WB,IP	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun
mouse	WB	Biochem Biophys Res Commun; Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.; Authors - Takumi Yokokawa