CEP112 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CEP112 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 24928-1-AP

Synonyme

CCDC46, Coiled-coil domain-containing protein 46, Centrosomal protein of 112 kDa

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  WB analysis of HEK-293 using 24928-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24928-1-AP (CEP112 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24928-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24928-1-AP (CEP112 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MDCK using 24928-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP112 antibody (24928-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

24928-1-AP bindet in WB, IF/ICC, ELISA CEP112 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP112 fusion protein Ag21586
• Vollständiger Name: coiled-coil domain containing 46
• Berechnetes Molekulargewicht: 955 aa, 113 kDa
• Beobachtetes Molekulargewicht: 110-113 kDa
• GenBank-Zugangsnummer: BC131805
• Gene symbol: CEP112
• Gene ID (NCBI): 201134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen ; KO Validated
mouse	WB,IF	PLoS Biol; Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.; Authors - Suchismita Panda
human	WB,IF	Clin Genet; Loss-of-function mutations in centrosomal protein 112 is associated with human acephalic spermatozoa phenotype.; Authors - Yanwei Sha

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (11-08-2019)

Human RPE1 cell line with Methanol fixationImmunostaining result shows that CEP112 localizes to centrosome.(Red;Cep112, Green; Cep164 for Centrosome marker, Blue;DAPI for Nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human RPE1

CEP112 Antibody Immunofluorescence, validation (1:100 dilution) in Human RPE1 (Cat no:24928-1-AP)