CWC25 Polyklonaler Antikörper

CWC25 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24416-1-AP

Synonyme

CCDC49, Spliceosome-associated protein homolog CWC25, Pre-mRNA-splicing factor CWC25 homolog, Coiled-coil domain-containing protein 49

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  WB analysis of HeLa using 24416-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24416-1-AP (CWC25 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24416-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24416-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 24416-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 24416-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 24416-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 24416-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24416-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CWC25 antibody (24416-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24416-1-AP bindet in WB, IHC, IF/ICC, ELISA CWC25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CWC25 fusion protein Ag17037
• Vollständiger Name: coiled-coil domain containing 49
• Berechnetes Molekulargewicht: 425 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC008833
• Gene symbol: CCDC49
• Gene ID (NCBI): 54883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.