CCDC56/COA3 Polyklonaler Antikörper

CCDC56/COA3 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20427-1-AP

Synonyme

CCDC56, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 12 kDa, HSPC009, Cytochrome c oxidase assembly factor 3 homolog, mitochondrial, Coiled-coil domain-containing protein 56

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  IHC staining of human colon cancer using 20427-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20427-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 20427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 20427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

20427-1-AP bindet in IHC, IF/ICC, ELISA CCDC56/COA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC56/COA3 fusion protein Ag14260
• Vollständiger Name: coiled-coil domain containing 56
• Berechnetes Molekulargewicht: 106 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC002698
• Gene symbol: CCDC56
• Gene ID (NCBI): 28958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human COX assembly factor 3 (COA3), also known as CCDC56 (coiled-coil domain-containing protein 56), is a mitochondrial transmembrane protein responsible for cytochrome c oxidase (COX) protein complex assembly. CCDC56/COA3 is located on chromosome 17 (17q.21.31) and encodes an 11.7-kDa protein with predicted transmembrane and coiled-coil domains (PMID: 22610097). CCDC56/COA3 is overexpressed at both mRNA and protein levels in human NSCLC cells, mainly as a result of decreased miR-338-3p level (PMID: 36119836).

Publikationen

Species	Application	Title
human	IHC	Am J Cancer Res; COA3 overexpression promotes non-small cell lung cancer metastasis by reprogramming glucose metabolism; Authors - Hongwei Lin