CCDC65 Polyklonaler Antikörper

CCDC65 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24376-1-AP

Synonyme

Testis development protein NYD-SP28, NYD SP28, Dynein regulatory complex subunit 2, DRC2, Coiled-coil domain-containing protein 65

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  WB analysis of mouse testis using 24376-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 24376-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24376-1-AP

  IP result of anti-CCDC65 (IP:24376-1-AP, 4ug; Detection:24376-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IF Staining of U2OS using 24376-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCDC65 antibody (24376-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

24376-1-AP bindet in WB, IF/ICC, IP, ELISA CCDC65 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC65 fusion protein Ag19357
• Vollständiger Name: coiled-coil domain containing 65
• Berechnetes Molekulargewicht: 484 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC039317
• Gene symbol: CCDC65
• Gene ID (NCBI): 85478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC65, also known as DRC2, is a sperm tail protein highly expressed in adult testis, spermatocytes, and spermatids. It is a component of the nexin-dynein regulatory complex (N-DRC), a structure important for regulating and orchestrating dynein activity. Mutations in CCDC65 are associated with primary ciliary dyskinesia (PCD), a genetic disorder that affects the structure and function of cilia(PMID: 23991085).

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.; Authors - Ziyan Zhang
human	IF	Andrology; A recurrent loss-of-function variant in DRC1 causes non-syndromic severe asthenozoospermia with favorable intracytoplasmic sperm injection and pregnancy outcomes; Authors - Célia Tebbakh