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CCDC76 Polyklonaler Antikörper

CCDC76 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31484-1-PBS

Synonyme

tRNA [Gm4] methyltransferase, TRMT13

Filter:
  • Western Blot (WB) analysis of various lysates using CCDC76 Polyclonal antibody (31484-1-AP)

    WB analysis using 31484-1-AP (same clone as 31484-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC76 Polyclonal antibody (31484-1-AP)

    IHC staining of human placenta using 31484-1-AP (same clone as 31484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CCDC76 Polyclonal antibody (31484-1-AP)

    IHC staining of mouse testis using 31484-1-AP (same clone as 31484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC76 Polyclonal antibody (31484-1-AP)

    IF Staining of HeLa using 31484-1-AP (same clone as 31484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31484-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CCDC76 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CCDC76 fusion protein Ag34110
Vollständiger Name coiled-coil domain containing 76
Beobachtetes Molekulargewicht54 kDa
GenBank-ZugangsnummerBC075811
Gene symbol CCDC76
Gene ID (NCBI) 54482
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).