CCDC85B Polyklonaler Antikörper

CCDC85B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18282-1-PBS

Synonyme

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  WB analysis of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 18282-1-AP (same clone as 18282-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

  IP result of anti-CCDC85B (IP:18282-1-AP, 4ug; Detection:18282-1-AP 1:300) with mouse heart tissue lysate 3600ug. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18282-1-AP (same clone as 18282-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18282-1-AP (CCDC85B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18282-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CCDC85B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC85B fusion protein Ag13129
• Vollständiger Name: coiled-coil domain containing 85B
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC008796
• Gene symbol: CCDC85B
• Gene ID (NCBI): 11007
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coiled-coil domain-containing protein 85B (CCDC85B), also named as DIPA, is 202 amino acid protein, which belongs to the CCDC85 family. CCDC85B localizes in the nucleus and cytoplasm. CCDC85B is widely expressed in many tissues and functions as a transcriptional repressor. CCDC85B may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. CCDC85B may play a role in adipocyte differentiation.