CCDC94 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CCDC94 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1G9

Kat-Nr. : 68324-1-Ig

Synonyme

YJU2, Coiled-coil domain-containing protein 94, 1D1G9

---

• 

  WB analysis using 68324-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68324-1-Ig (CCDC94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HeLa using 68324-1-Ig

  WB result of CCDC94 antibody (68324-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

• 

  IF Staining of A431 using 68324-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC94 antibody (68324-1-Ig, Clone: 1D1G9 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68324-1-Ig bindet in WB, IF/ICC, ELISA CCDC94 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CCDC94 fusion protein Ag13772
• Vollständiger Name: coiled-coil domain containing 94
• Berechnetes Molekulargewicht: 323 aa, 37 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC000561
• Gene symbol: CCDC94
• Gene ID (NCBI): 55702
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)