CCDC99 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CCDC99 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 24689-1-AP

Synonyme

CCDC99, hSpindly, MU-RMS-40.4A, Protein Spindly, SPDL1, spdly

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• 

  WB analysis using 24689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24689-1-AP

  WB result of CCDC99 antibody (24689-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC99 transfected HeLa cells.

• 

  IP experiment of HEK-293 using 24689-1-AP

  IP result of anti-CCDC99 (IP:24689-1-AP, 4ug; Detection:24689-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human liver cancer using 24689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

24689-1-AP bindet in WB, IHC, IF, IP, ELISA CCDC99 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC99 fusion protein Ag20430
• Vollständiger Name: coiled-coil domain containing 99
• Berechnetes Molekulargewicht: 605 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC012568
• Gene symbol: CCDC99
• Gene ID (NCBI): 54908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IF	Cells; Identification of Ku70 Domain-Specific Interactors Using BioID2.; Authors - Sanna Abbasi
human	WB	PLoS One; Inhibiting the expression of spindle appendix cooled coil protein 1 can suppress tumor cell growth and metastasis and is associated with cancer immune cells in esophageal squamous cell carcinoma; Authors - Tao Liu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Martijn (Verified Customer) (03-02-2023)

Antibody seems to work really well for western blotting, the signal is strong, and it doesn't show a lot of background. For immunofluorescence it does not give a clear signal, and it looks like it only shows background

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB: 1:1000, IF: 1:100
• Cell Tissue Type: HeLa

CCDC99 Antibody Western Blot,Immunofluorescence validation (WB: 1:1000, IF: 1:100 dilution) in HeLa (Cat no:24689-1-AP)