CCKAR-specific Rekombinanter Antikörper

CCKAR-specific Rekombinant Antikörper für WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242681A12

Kat-Nr. : 85205-1-PBS

Synonyme

CCKAR, 242681A12, CCK A, CCK A receptor, CCK AR

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  WB analysis using 85205-1-RR (same clone as 85205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85205-1-RR (CCKAR-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01958-1

  Sandwich ELISA standard curve of MP01958-1, Human CCKAR Recombinant Matched Antibody Pair - PBS only. 85205-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00105. 85205-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01958-1

  The mean CCKAR concentration was determined to be 4.0 ng/mL in HUVEC cell extract based on a 0.9 mg/mL extract load and 5.3 ng/mL in SGC-7901 cell extract based on a 2.4 mg/mL extract load.

• 

  IP experiment of mouse lung using 85205-1-RR (same clone as 85205-1-PBS)

  IP result of anti-CCKAR-specific (IP:85205-1-RR, 4ug; Detection:85205-1-RR 1:600) with mouse lung tissue lysate 1520 ug. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85205-1-PBS bindet in WB, IP, Sandwich ELISA, Indirect ELISA, Sample test CCKAR-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cholecystokinin A receptor
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_000730
• Gene symbol: CCKAR
• Gene ID (NCBI): 886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.