CCL11/Eotaxin Polyklonaler Antikörper

CCL11/Eotaxin Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11786-1-AP

Synonyme

CCL11, Eotaxin, Eosinophil chemotactic protein, CCL-11, CCL 11

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  IHC staining of human lung squamous cell carcinoma using 11786-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 11786-1-AP (Eotaxin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung squamous cell carcinoma using 11786-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 11786-1-AP (Eotaxin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11786-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11786-1-AP (Eotaxin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11786-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11786-1-AP (Eotaxin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: human lung squamous cell carcinoma tissue, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

11786-1-AP bindet in IHC, ELISA CCL11/Eotaxin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCL11/Eotaxin fusion protein Ag2398
• Vollständiger Name: chemokine (C-C motif) ligand 11
• Berechnetes Molekulargewicht: 97 aa, 11 kDa
• GenBank-Zugangsnummer: BC017850
• Gene symbol: CCL11/Eotaxin
• Gene ID (NCBI): 6356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL11 also named Eotaxin-1, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.

Publikationen

Species	Application	Title
mouse	IHC	Am J Physiol Heart Circ Physiol; Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.; Authors - Li Lin
rat	IHC	Am J Rhinol Allergy; Effects of Ursolic Acid on the Expression of Th1-Th2-related Cytokines in a Rat Model of Allergic Rhinitis After PM2.5 Exposure.; Authors - Na Sun