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CCL12/MCP-5 Rekombinanter Antikörper

CCL12/MCP-5 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250386A9

Kat-Nr. : 86565-1-PBS

Synonyme

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

Filter:
  • Western Blot (WB) analysis of various lysates using CCL12/MCP-5 Recombinant antibody (86565-1-RR)

    WB analysis using 86565-1-RR (same clone as 86565-1-PBS)

    Non-transfected and CCL12-transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 86565-1-RR (Ccl12 antibody) at a dilution of 1:5000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86565-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86565-1-PBS bindet in WB, Indirect ELISA CCL12/MCP-5 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CCL12/MCP-5 fusion protein Eg3315
Vollständiger Name chemokine (C-C motif) ligand 12
Berechnetes Molekulargewicht12KD
GenBank-ZugangsnummerNM_011331.2
Gene symbol Ccl12
Gene ID (NCBI) 20293
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.