CCL12/MCP-5 Rekombinanter Antikörper

CCL12/MCP-5 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250386A9

Kat-Nr. : 86565-1-RR

Synonyme

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

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  WB analysis using 86565-1-RR

  Non-transfected and CCL12-transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 86565-1-RR (Ccl12 antibody) at a dilution of 1:5000, incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfected with Mouse Ccl12 Transfected HEK-293T cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86565-1-RR bindet in WB, ELISA CCL12/MCP-5 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL12/MCP-5 fusion protein Eg3315
• Vollständiger Name: chemokine (C-C motif) ligand 12
• Berechnetes Molekulargewicht: 12KD
• GenBank-Zugangsnummer: NM_011331.2
• Gene symbol: Ccl12
• Gene ID (NCBI): 20293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.