CCL20/MIP-3 alpha Rekombinanter Antikörper

CCL20/MIP-3 alpha Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241694B4

Kat-Nr. : 84413-3-RR

Synonyme

CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20

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  WB analysis using 84413-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84413-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 84413-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HepG2-Zellen, HuH-7-Zellen, RAW 264.7-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1250-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84413-3-RR bindet in WB, IHC, ELISA CCL20/MIP-3 alpha und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: chemokine (C-C motif) ligand 20
• Berechnetes Molekulargewicht: 11kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_004591.3
• Gene symbol: CCL20/MIP-3 alpha
• Gene ID (NCBI): 6364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.