CCL20/MIP-3 alpha Rekombinanter Antikörper

CCL20/MIP-3 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241694B2

Kat-Nr. : 98221-1-PBS

Synonyme

CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20

---

• 

  FC experiment of THP-1 using 98221-1-RR (same clone as 98221-1-PBS)

  1x10^6 PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 0.25 ug Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody (98221-1-RR, Clone: 241694B2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated THP-1 cells were intracellularly stained with 0.25 ug Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody (98221-1-RR, Clone: 241694B2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98221-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

98221-1-PBS bindet in FC (Intra) CCL20/MIP-3 alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: chemokine (C-C motif) ligand 20
• Berechnetes Molekulargewicht: 11kDa
• GenBank-Zugangsnummer: NM_004591.3
• Gene symbol: CCL20/MIP-3 alpha
• Gene ID (NCBI): 6364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.