CCL24/Eotaxin 2 Polyklonaler Antikörper

CCL24/Eotaxin 2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 22306-1-AP

Synonyme

CCL24, Eotaxin 2, CK-beta-6, CCL-24, CCL 24

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  WB analysis of mouse thymus using 22306-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

22306-1-AP bindet in WB, IHC, IF, ELISA CCL24/Eotaxin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: chemokine (C-C motif) ligand 24
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: NM_002991
• Gene symbol: CCL24/Eotaxin 2
• Gene ID (NCBI): 6369
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chemokine CCL24 is the second member of eotaxins, a group of eosinophils' selectively chemoattractants. CCL24 is constitutively expressed in fibroblasts, epithelial cells, macrophages. CCL24 has only one receptor CCR3, which mainly presents on eosinophils and was also found on basophils, monocytes, Th2 lymphocytes, epithelial cells and airway smooth muscles. CCL24 displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes.

Publikationen

Species	Application	Title
		Clin Transl Med; NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization; Authors - Shaomin Zou
mouse	WB	Dis Markers; CCL24 Protects Renal Function by Controlling Inflammation in Podocytes.; Authors - Youdi Wang
human,mouse	IHC,IF	Cell Death Dis; Mechanical stiffness promotes skin fibrosis via Piezo1-Wnt2/Wnt11-CCL24 positive feedback loop; Authors - Jiahao He