CCL28 Polyklonaler Antikörper

CCL28 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18214-1-PBS

Synonyme

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  WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 18214-1-AP (same clone as 18214-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18214-1-AP (same clone as 18214-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 18214-1-AP (same clone as 18214-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18214-1-PBS bindet in WB, IHC, Indirect ELISA CCL28 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCL28 fusion protein Ag12920
• Vollständiger Name: chemokine (C-C motif) ligand 28
• Berechnetes Molekulargewicht: 127 aa, 14 kDa
• Beobachtetes Molekulargewicht: 8 kDa-9 kDa
• GenBank-Zugangsnummer: BC069532
• Gene symbol: CCL28
• Gene ID (NCBI): 56477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).