CCL3/MIP-1 alpha Rekombinanter Antikörper

CCL3/MIP-1 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241695B10

Kat-Nr. : 98194-1-PBS

Synonyme

241695B10, C-C motif chemokine 3, Ccl3, Heparin-binding chemotaxis protein, L2G25B

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  FC experiment of J774A.1 using 98194-1-RR (same clone as 98194-1-PBS)

  1x10^6 LPS and Brefeldin A treated J774A.1 cells were intracellularly stained with 0.25 ug Anti-Mouse CCL3 Rabbit Recombinant Antibody (98194-1-RR, Clone:241695B10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated J774A.1 cells were intracellularly stained with 0.25 ug Anti-Mouse CCL3 Rabbit Recombinant Antibody (98194-1-RR, Clone: 241695B10) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98194-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98194-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98194-1-RR against Mouse CCL3 were performed. The affinity constant is 3.87 nM.

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Geprüfte Anwendungen

Produktinformation

98194-1-PBS bindet in FC (Intra) CCL3/MIP-1 alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL3/MIP-1 alpha fusion protein Eg2170
• Vollständiger Name: chemokine (C-C motif) ligand 3
• Berechnetes Molekulargewicht: 10kd
• GenBank-Zugangsnummer: NM_011337
• Gene symbol: Ccl3
• Gene ID (NCBI): 20302
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.