CCL4/MIP-1 beta Polyklonaler Antikörper

CCL4/MIP-1 beta Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26614-1-AP

Synonyme

ACT 2, ACT2, AT744.1, C C motif chemokine 4, CCL4, chemokine (C C motif) ligand 4, G 26, HC21, LAG 1, LAG1, MIP 1 beta, MIP 1 beta(1 69), MIP-1 beta, MIP1B, MIP1B1, MIP-1β, PAT 744, Protein H400, SCYA2, SCYA4, SIS gamma, Small inducible cytokine A4, T cell activation protein 2

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  WB analysis using 26614-1-AP

  Untreated THP-1 cells, PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 26614-1-AP (MIP 1 beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 26614-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 26614-1-AP (MIP 1 beta antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, LPS and Brefeldin A treated THP-1 cells, Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26614-1-AP bindet in WB, ELISA CCL4/MIP-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCL4/MIP-1 beta fusion protein Ag25070
• Vollständiger Name: chemokine (C-C motif) ligand 4
• Berechnetes Molekulargewicht: 92 aa, 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC107433
• Gene symbol: CCL4/MIP-1 beta
• Gene ID (NCBI): 6351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. MIP-1β is an acidic protein composed of 69 amino acids that is produced by many cells, particularly macrophages, dendritic cells, and lymphocytes. MIP-1β is responsible for the activation of PMN and is involved in acute neutrophilic inflammation. Recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).