RANTES Polyklonaler Antikörper

RANTES Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, Neutralization, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 12000-1-AP

Synonyme

C C motif chemokine 5, CCL5, chemokine (C C motif) ligand 5, D17S136E, EoCP, RANTES, SCYA5, SIS delta, SISd, Small inducible cytokine A5, T cell specific protein P228, T cell specific protein RANTES, TCP228

---

• 

  WB analysis using 12000-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12000-1-AP (RANTES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Produktinformation

12000-1-AP bindet in WB, IHC, IF, Neutralization, ELISA RANTES und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RANTES fusion protein Ag2617
• Vollständiger Name: chemokine (C-C motif) ligand 5
• Berechnetes Molekulargewicht: 91 aa, 10 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: BC008600
• Gene symbol: CCL5/RANTES
• Gene ID (NCBI): 6352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RANTES (CCL5) belongs to the CC chemokine family and induces leukocyte migration by binding to specific receptors in the G protein-coupled receptor family. RANTES (CCL5) has been shown in vitro to mediate eosinophil, lymphocyte, neutrophil, and monocyte chemotaxis, and it initiates several other proinflammatory events, such as integrin activation, lipid mediator biosynthesis, and degranulation.

Publikationen

Species	Application	Title
mouse	WB	Brain Behav Immun; Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease; Authors - Jiaxin Guan
rat	WB	Phytomedicine; Integration of transcriptomics and metabolomics reveals the mechanism of Glycyrrhizae Radix Et Rhizoma extract inhibiting CCL5 in the treatment of acute pharyngitis; Authors - Xiaoxiao Han
mouse	IHC	Immunology; NME4 suppresses NFκB2-CCL5 axis, restricting CD8+ T cell tumour infiltration in oesophageal squamous cell carcinoma; Authors - Shutao Zheng
		iScience; Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis; Authors - Zhi Xiong
mouse	WB,IF	Stem Cells; Targeted deletion of Rictor in BMSCs reduces the biological activity of K7M2 cells and mitigates OS-induced bone destruction; Authors - Jinhong Lu
human	Neutralization	Ann Transl Med; Upregulation of STAT1-CCL5 axis is a biomarker of colon cancer and promotes the proliferation of colon cancer cells.; Authors - Mengke Niu