CCL7/MCP-3 Rekombinanter Antikörper

CCL7/MCP-3 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250662G6

Kat-Nr. : 98555-1-PBS

Synonyme

C-C motif chemokine 7, Ccl7, Fic, Intercrine/chemokine MARC, Mcp3

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  FC experiment of Transfected HEK-293T using 98555-1-RR (same clone as 98555-1-PBS)

  1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone: 250662G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98555-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98555-1-RR (same clone as 98555-1-PBS)

  1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone:250662G6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98555-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98555-1-PBS bindet in FC (Intra) CCL7/MCP-3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL7/MCP-3 fusion protein Eg4626
• Vollständiger Name: chemokine (C-C motif) ligand 7
• Berechnetes Molekulargewicht: 11kDa
• GenBank-Zugangsnummer: NM_013654.3
• Gene symbol: Ccl7
• Gene ID (NCBI): 20306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10.