CCL7/MCP-3 Rekombinanter Antikörper

CCL7/MCP-3 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250662G6

Kat-Nr. : 98555-1-RR

Synonyme

C-C motif chemokine 7, Ccl7, Fic, Intercrine/chemokine MARC, Mcp3

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  FC experiment of Transfected HEK-293T using 98555-1-RR

  1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone: 250662G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of Transfected HEK-293T using 98555-1-RR

  1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone:250662G6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98555-1-RR bindet in FC (Intra) CCL7/MCP-3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL7/MCP-3 fusion protein Eg4626
• Vollständiger Name: chemokine (C-C motif) ligand 7
• Berechnetes Molekulargewicht: 11kDa
• GenBank-Zugangsnummer: NM_013654.3
• Gene symbol: Ccl7
• Gene ID (NCBI): 20306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10.