CCL8/MCP-2 Rekombinanter Antikörper

CCL8/MCP-2 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250174H6

Kat-Nr. : 98480-1-PBS

Synonyme

Ccl8, C-C motif chemokine 8, Ccl 8, CCL-8, chemokine (C C motif) ligand 8

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  FC experiment of Transfected HEK-293T using 98480-1-RR (same clone as 98480-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL8/MCP-2-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL8/MCP-2 Rabbit RecAb (98480-1-RR, Clone: 250174H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98480-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98480-1-RR (same clone as 98480-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL8/MCP-2-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL8/MCP-2 Rabbit RecAb (98480-1-RR, Clone: 250174H6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98480-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98480-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98480-1-RR against Mouse CCL8/MCP-2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98480-1-PBS bindet in FC (Intra) CCL8/MCP-2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL8/MCP-2 fusion protein Eg2771
• Vollständiger Name: chemokine (C-C motif) ligand 8
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_021443.3
• Gene symbol: Ccl8
• Gene ID (NCBI): 20307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

The cys-cys (C-C) motif chemokine ligand 8 (CCL8, also known as MCP2) is implicated in various inflammatory processes. In bone marrow transplantation, circulating CCL8 levels positively correlate with the severity of graft-versus-host disease.