CCL9 Rekombinanter Antikörper

CCL9 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

243103H5

Kat-Nr. : 98475-1-PBS

Synonyme

C-C motif chemokine 9, CCF18, CCL9(29-101), CCL9(30-101), CCL9(31-101)

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  FC experiment of Transfected HEK-293T using 98475-1-RR (same clone as 98475-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone: 243103H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98475-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98475-1-RR (same clone as 98475-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone:243103H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98475-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98475-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98475-1-RR against Mouse CCL9 were performed. The affinity constant is 0.649 nM.

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Geprüfte Anwendungen

Produktinformation

98475-1-PBS bindet in FC (Intra) CCL9 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCL9 fusion protein Eg3336
• Vollständiger Name: chemokine (C-C motif) ligand 9
• Berechnetes Molekulargewicht: 14KD
• GenBank-Zugangsnummer: NM_011338.2
• Gene symbol: Ccl9
• Gene ID (NCBI): 20308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Mouse C-C motif ligand 9 (CCL9), alternatively named macrophage inflammatory protein 1γ (MIP-1γ), was identified in 1995 and is homologous to the mouse CCL6, as well as human CCL23 and CCL15. It is noted that CCL9 is also known by various names such as macrophage inflammatory protein-1 gamma (MIP-1ɣ), macrophage inflammatory protein-related protein-2 (MRP-2) and CCF18 in rodents. Monocytes and myeloid cell lines produce large quantities of CCL9, as do dendritic cells and T cells, in particular Th1 type T cells. Despite high baseline levels in circulation, it has become apparent that concentrations of CCL9 vary greatly in specific tissues with profound effects on health. For example, in the bone, CCL9 is produced at even higher levels, and is critical to osteoclast versus osteoblast differentiation of macrophages. There are also indications of a timed specific induction of CCL9 in skin wound healing and follicle-associated epithelium of the gut.