Cyclin G Polyklonaler Antikörper

Cyclin G Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10897-1-AP

Synonyme

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 10897-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10897-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10897-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10897-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10897-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, HeLa-Zellen, Maus-Eierstockgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

10897-1-AP bindet in WB, IHC, ELISA Cyclin G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin G fusion protein Ag1157
• Vollständiger Name: cyclin G1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC007093
• Gene symbol: CCNG1
• Gene ID (NCBI): 900
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1.; Authors - Mingjing Yan
human	WB	Cancer Lett; The C/EBPβ-LINC01133 axis promotes cell proliferation in pancreatic ductal adenocarcinoma through upregulation of CCNG1.; Authors - Chen-Song Huang
human	WB	J Biol Chem; MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.; Authors - Fanpeng Zhao
human	WB	Exp Cell Res; PRMT6 functionally associates with PRMT5 to promote colorectal cancer progression through epigenetically repressing the expression of CDKN2B and CCNG1; Authors - Yuzhong Chen
human	WB	Oncol Res; Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2; Authors - Yusen Zhang