Cyclin T1 Polyklonaler Antikörper

Cyclin T1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20992-1-AP

Synonyme

CCNT, CCNT1, Cyclin T, cyclin T1, CYCT1, HIVE1

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Galerie der Validierungsdaten

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  WB analysis using 20992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20992-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20992-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20992-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 20992-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20992-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20992-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 20992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin T1 antibody (20992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

20992-1-AP bindet in WB, IF, ELISA Cyclin T1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cyclin T1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: NM_001240
• Gene symbol: CCNT1
• Gene ID (NCBI): 904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB,IF	BMC Cancer; The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study; Authors - Ying E