CCNY Polyklonaler Antikörper

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• KD/KO Validated

CCNY Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 18042-1-AP

Synonyme

C10orf9, CBCP1, CFP1, Cyc Y, Cyclin box protein 1

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  WB analysis using 18042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18042-1-AP (CCNY antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18042-1-AP

  WB result of CCNY antibody (18042-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNY transfected A549 cells.

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  IP experiment of K-562 using 18042-1-AP

  IP result of anti-CCNY (IP:18042-1-AP, 4ug; Detection:18042-1-AP 1:2000) with K-562 cells lysate 1600 ug.

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  IHC staining of mouse testis using 18042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18042-1-AP (CCNY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 18042-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18042-1-AP (CCNY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 18042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCNY antibody (18042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 18042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCNY antibody (18042-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

18042-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CCNY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCNY fusion protein Ag12674
• Vollständiger Name: cyclin Y
• Berechnetes Molekulargewicht: 33 kDa, 39 kDa
• Beobachtetes Molekulargewicht: 33-39 kDa
• GenBank-Zugangsnummer: BC104773
• Gene symbol: CCNY
• Gene ID (NCBI): 219771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.

Publikationen

Species	Application	Title
rat	WB	Prog Neurobiol; Cyclin Y, a novel actin-binding protein, regulates spine plasticity through the cofilin-actin pathway.; Authors - Hongik Hwang
mouse,human	WB,IP	Prog Neurobiol; Myristoylation-dependent palmitoylation of cyclin Y modulates long-term potentiation and spatial learning; Authors - Jiyeon Seo
mouse	WB	Pharmacol Res; Ccny knockout mice display an enhanced susceptibility to kainic acid-induced epilepsy.; Authors - Hongik Hwang ; KO Validated
mouse	WB	Biochem J; Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16.; Authors - Saifeldin N Shehata
human	WB,IF	Cells; The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis.; Authors - Andrea Molinas
mouse	WB,IP	Cell Signal; Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase.; Authors - Shehata Saifeldin N SN